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Medición de la deformabilidad y la heterogeneidad de glóbulos rojos en sangre por Ektacytometry
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Pediatrics,Saint Louis University School of Medicine, 2Molecular and Clinical Nutrition Section, Digestive Diseases Branch,National Institute of Diabetes and Digestive and Kidney Diseases, 3Molecular and Clinical Hematology Branch,National Institute of Diabetes and Digestive and Kidney Diseases, 4Sickle Cell Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 5Edward A. Doisy Department of Biochemistry and Molecular Biology,Saint Louis University School of Medicine, 6Red Cell Physiology Laboratory,New York Blood Center
Chapters
- 00:05Title
- 01:05Ektacytometer Set-up
- 01:48Measuring Deformability as a Function of Increasing Shear Stress
- 04:10Measuring Cellular Heterogeneity
- 05:20Osmotic Gradient Ektacytometry
- 07:16Results: Diffraction Pattern Distortion and Relationship to Hemoglobin
- 08:41Conclusion
Aquí presentamos técnicas para medir la deformabilidad del glóbulo rojo y la heterogeneidad celular por ektacytometry. Estas técnicas son aplicables a las investigaciones generales de la deformabilidad del glóbulo rojo y las investigaciones específicas de enfermedades de la sangre caracterizadas por la presencia de rígidos y deformables eritrocitos en la circulación, tales como anemia de células falciformes.
