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JoVE Journal Bioengineering
Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies
 

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Article DOI: 10.3791/56924-v • 08:10 min • March 21st, 2019
March 21st, 2019 •
1,2,3,4, 1,2,5, 4,6, 1,2,3, 1,2, 1,2,3
1i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 2INEB - Instituto de Engenharia Biomédica, Universidade do Porto, 3Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, 4Gladstone Institute of Cardiovascular Disease, 5Faculty of Medicine, University of Porto, 6University of California San Francisco

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요약
Automatic Translation

The cardiac extracellular matrix (ECM) is a complex network of molecules that orchestrate key processes in tissues and organs while enduring physiological remodeling throughout life. Standardized decellularization of fetal and adult hearts permits comparative experimental studies of both tissues in a 3D context by capturing native architecture and biomechanical properties.

Tags

Keywords: Decellularization Fetal Cardiac Tissue Adult Cardiac Tissue Extracellular Matrix Comparative Analysis 3D-like Platforms In Vitro Studies PBS OCT Compound Hypotonic Buffer Sodium Dodecyl Sulfate
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