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1Department of Environmental Sciences and Engineering, Gillings School of Global Public Health,University of North Carolina at Chapel Hill, 2Environmental Public Health Division,National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education
Chapters
- 00:04Title
- 00:40Microscopy Set-up
- 05:09Data Acquisition
- 07:36Results: Toxicological Assessment of Changes in the Intracellular EGSH as Measured by roGFP2
- 08:54Conclusion
Dieses Manuskript beschreibt die Verwendung von genetisch codierte Fluorogenic Reporter in einer live Cell Imaging-Anwendung für die Prüfung der Harnblase-induzierten oxidativen Stress. Diese experimentelle Ansatz bietet unvergleichliche räumlich-zeitliche Auflösung, Sensitivität und Spezifität vieler die Unzulänglichkeiten der konventionellen Methoden zur Erkennung von toxikologischen oxidativen Stress zu vermeiden.
