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Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls
DOI:

10:16 min

January 04, 2018

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Chapters

  • 00:05Title
  • 00:51Jugular Catheter Implantation and Carotids Preparation
  • 05:11Assessment of Rolling and Adhering Leukocytes/LysM+ Cells with Intravital Video Microscopy
  • 07:29Results: Leukycote Rolling and Adhesion in Angiotensin II-Infused Mice
  • 09:18Conclusion

Summary

자동 번역

This manuscript describes the use of transgenic reporter mice and different administration routes of fluorescent dyes in angiotensin II-induced hypertension using intravital video microscopy of blood vessels to evaluate the activation of immune cells and their ability to roll and adhere to the endothelium.

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