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면역학 및 감염병학

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中性粒细胞衍生微粒的分离与鉴定功能研究

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

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中性粒细胞衍生微粒的分离与鉴定功能研究

DOI:

10.3791/56949-v

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06:56 min

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March 02, 2018

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Ariel Finkielsztein, Lorraine Mascarenhas, Veronika Butin-Israeli, Ronen Sumagin

¹Department of Pathology, Feinberg School of Medicine,Northwestern University

Chapters

00:05Title
00:55Murine Bone Marrow Polymorphonuclear (PMN) Cell Separation
02:37Microparticle (MP) Isolation from Bone Marrow-derived Activated Murine PMNs
03:45In Vivo PMN-derived Microparticle (MP) Wound Healing Assay
04:38Results: Representative Effects of Isolated PMN-MPs in Epithelial Wound Healing
05:36Conclusion

Summary

자동 번역

本文介绍了新的协议, 以分离和表征从人和小鼠中性粒细胞衍生的微粒。这些协议利用离心、流式细胞术和免疫印迹法技术对微粒含量进行分析, 可用于研究不同细胞类型的微粒在细胞功能中的作用。

Tags

Neutrophil-derived Microparticles Cell-cell Communication Density Gradient Separation Polymorphonuclear Cells PMN Mononuclear Cells Cell-free Supernatants Ultra-centrifugation Functional Studies

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