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Ensayos de flujo laminar para investigar el reclutamiento leucocitario en cultivos de células vasculares y las plaquetas adherentes
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
DOI:

08:50 min

April 09, 2018

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Chapters

  • 00:05Title
  • 00:57Flow-based Assay with Human Cells
  • 02:28Preparation of Adherent Platelet and Vascular Cell Monolayers
  • 03:56Fluorescent Labeling and Perfusion of Leukocytes
  • 06:32Results: Use of Laminar Flow-based Assay to Investigate the Interactions of Leukocytes with Their Cellular Partners
  • 08:09Conclusion

Summary

자동 번역

Leucocitos con avidez interactúan con las células vasculares y las plaquetas después de lesión de pared del recipiente o durante la inflamación. Aquí, describimos un análisis sencillo basado en el flujo laminar para caracterizar los mecanismos moleculares que subyacen a las interacciones entre los leucocitos y sus socios celulares.

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