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1Institute of Chemistry L 1, Department of Biocatalysis,Technical University of Berlin, 2Institute of Chemistry PC 14, Department of Bioenergetics,Technical University of Berlin, 3Institute of Chemistry TC 7, Department of Physical Chemistry/Molecular Material Sciences,Technical University of Berlin
Chapters
- 00:05Title
- 01:46Recombinant Protein Expression
- 04:05Target Protein Purification via Immobilized Metal Ion Affinity Chromatography
- 05:20Intact Protein Mass Analysis by High-performance Liquid Chromatography Coupled to Electrospray Ionization Time-of-flight Mass Spectrometry
- 06:38Fluorescence Lifetime Measurements and Decay-associated Spectra of GdFP
- 07:59Results: Gold Fluorescent Protein Analysis by Mass Spectrometry and Fluorescence
- 10:13Conclusion
Sentetik Biyoloji Mühendisliği proteinlerin kanonik olmayan amino asitlerin co-translational ekleme kullanarak benzersiz özellikleri ile sağlar. Burada, GFP türü fluorophore "altın" floresan protein (GdFP) olarak adlandırdığı roman Floresans spektroskopik özellikleri ile hayalice kırmızı kaymıştır bir türevi E. coli seçici basınç birleşme (SPI) yolu ile nasıl üretilir sundu.
