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1Department of Medicine, Division of Hematology,Johns Hopkins University School of Medicine, 2Department of Natural Sciences, School of Arts & Sciences,Lebanese American University, 3Department of Cell Biology,Johns Hopkins University School of Medicine, 4Department of Oncology,Johns Hopkins University School of Medicine, 5Department of Pathology,Johns Hopkins University School of Medicine, 6Institute for Cell Engineering,Johns Hopkins University School of Medicine
Chapters
- 00:04Title
- 01:29Genetic Engineering of Organoids by Viral Transduction
- 02:26Seeding of Infected Organoid Fragments
- 03:44Organoid Cryosection in Basement Membrane Matrix
- 05:32Results: Efficient Viral Transduction of Intestinal Organoids
- 06:21Conclusion
We beschrijven stap-voor-stap instructies voor: 1) efficiënt ingenieur intestinale organoïden met behulp van magnetische nanodeeltjes voor Lenti-of retrovirale transductie, en, 2) het genereren van bevroren secties van engineered organoïden. Deze aanpak biedt een krachtig hulpmiddel om de genexpressie in organoïden efficiënt te veranderen voor onderzoek naar stroomafwaartse effecten.
