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Journal / Immunology and Infection / 人精切肺切片中物质的细胞毒性及免疫调节作用的评价…
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Immunology and Infection

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JoVE Journal Immunology and Infection
人精切肺切片中物质的细胞毒性及免疫调节作用的评价
 
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人精切肺切片中物质的细胞毒性及免疫调节作用的评价

Article DOI: 10.3791/57042-v • 12:04 min • May 9th, 2018
May 9th, 2018 •
*1, *1, 1, 1, 1, 2, 2, 3, 4, 4, 5, 1,6, 1
1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School
* These authors contributed equally

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鉴于3Rs 原则, 呼吸道模型作为动物研究的替代品正在演变。特别是对于呼吸物质的风险评估, 缺乏适当的化验方法。在这里, 我们描述了使用人的精密切割肺切片评估机载物质。

Tags

免疫学和感染 问题 135 人体外植体培养 脊髓组织 活检 肺材料 体外 细胞毒性 免疫 共焦显微术 化学药品 精确切肺切片
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