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Journal / Immunology and Infection / Bedömning av den cytotoxiska och immunmodulerande …
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Bedömning av den cytotoxiska och immunmodulerande effekter av ämnen i Human Precision-cut Lung skivor
 
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Bedömning av den cytotoxiska och immunmodulerande effekter av ämnen i Human Precision-cut Lung skivor

Article DOI: 10.3791/57042-v • 12:04 min • May 9th, 2018
May 9th, 2018 •
*1, *1, 1, 1, 1, 2, 2, 3, 4, 4, 5, 1,6, 1
1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School
* These authors contributed equally

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요약
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Med tanke på 3R-principen utvecklas respiratorisk modeller som alternativ till djurförsök. Särskilt för riskbedömning av respiratoriska ämnen finns det en brist på lämpliga analyser. Här, beskriver vi användning av mänsklig precision-cut lung skivor för bedömningen av luftburna ämnen.

Tags

Immunologi och infektion fråga 135 mänskliga explant kultur organotypic vävnad biopsi lung material ex vivo cytotoxicitet immunmodulering konfokalmikroskopi kemikalier precision-cut lung skivor
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