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1Department of Cellular and Integrative Physiology,Indiana University School of Medicine, 2Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, 3Indiana Center for Biological Microscopy,Indiana University School of Medicine, 4Department of Medicine,Indiana University School of Medicine, 5Department of Pediatrics,Indiana University School of Medicine, 6Department of Microbiology and Immunology,Indiana University School of Medicine, 7Indiana University Simon Cancer Center,Indiana University School of Medicine
Chapters
- 00:05Title
- 00:59Microscope Preparation and Endothelial Colony Forming Cell (ECFC) Matrix Plating
- 04:46Image Acquisition and Processing and Kinetic Analysis of Vasculogenesis (KAV)
- 08:12Results: Representative Kinetic Analysis of Vasculogenesis
- 10:22Conclusion
Ici, nous présentons un protocole pour l’imagerie Time-lapse et analyse de vasculogénèse in vitro à l’aide couplé avec le logiciel open source, analyse cinétique de vasculogénèse au microscope à contraste de phase. Ce protocole peut être appliqué pour évaluer quantitativement le potentiel vasculogenic de nombreux types de cellules ou des conditions expérimentales qui modélisent les maladies vasculaires.
