This content is Free Access.
1Department of Cellular and Integrative Physiology,Indiana University School of Medicine, 2Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, 3Indiana Center for Biological Microscopy,Indiana University School of Medicine, 4Department of Medicine,Indiana University School of Medicine, 5Department of Pediatrics,Indiana University School of Medicine, 6Department of Microbiology and Immunology,Indiana University School of Medicine, 7Indiana University Simon Cancer Center,Indiana University School of Medicine
Chapters
- 00:05Title
- 00:59Microscope Preparation and Endothelial Colony Forming Cell (ECFC) Matrix Plating
- 04:46Image Acquisition and Processing and Kinetic Analysis of Vasculogenesis (KAV)
- 08:12Results: Representative Kinetic Analysis of Vasculogenesis
- 10:22Conclusion
Här presenterar vi ett protokoll för time-lapse imaging och analys av vasculogenesis in vitro- använda fas kontrast mikroskopi tillsammans med öppen källkod, Kinetic analys av Vasculogenesis. Detta protokoll kan tillämpas för att bedöma kvantitativt vasculogenic potentialen i många celltyper eller experimentella förhållanden som modellerar vaskulär sjukdom.
