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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
JoVE 신문
생화학
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JoVE 신문 생화학
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
DOI:

06:18 min

March 13, 2018

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Chapters

  • 00:05Title
  • 00:55RT-LAMP for Detection of Zika-infected Mosquitoes Using Q-paper Technology
  • 02:23Testing Lyophilized RT-LAMP Reagents on Urine Samples Containing Zika
  • 04:05Results: Singleplex and Multiplex RT-LAMP for Detection of Zika, Chikungunya, and Dengue 1
  • 05:40Conclusion

Summary

자동 번역

Current multiplexed diagnostics to detect Zika, chikungunya, and dengue viruses require complex sample preparation and expensive instrumentation, and are difficult to use in low resource environments. We show a diagnostic that uses isothermal amplification with target-specific strand displaceable probes to detect and differentiate these viruses with high sensitivity and specificity.

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