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Chapters
- 00:04Title
- 00:37RNA Sample Preparation
- 02:37Immunoprecipitation Bead Preparation
- 04:51Binding BrU-labelled RNA to Beads
- 05:46RNA Elution Purification
- 08:29Results: Increase in Steady State RNA Caused by Increase in RNA Synthesis
- 09:35Conclusion
This method can be used to measure RNA synthesis. 5-Bromouridine is added to cells and incorporated into synthesized RNA. RNA synthesis is measured by RNA extraction immediately after labelling, followed by 5-Bromouridine-targeted immunoprecipitation of labelled RNA and analysis by reverse transcription and quantitative polymerase chain reaction.
