/
/
Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:45Ex Vivo Infection
- 01:48Hemolymph Extraction
- 03:11In Vivo Infection
- 04:08Hemolymph Extraction and Hemocyte Plating
- 04:35Confocal Imaging
- 05:443D Model Reconstruction
- 06:58Results: Dynamic Visualization of Drosophila Hemocytes
- 08:09Conclusion
This method demonstrates how to visualize pathogen invasion into insect cells with three-dimensional (3D) models. Hemocytes from Drosophila larvae were infected with viral or bacterial pathogens, either ex vivo or in vivo. Infected hemocytes were then fixed and stained for imaging with a confocal microscope and subsequent 3D cellular reconstruction.
