JoVE Journal
Biology
Biology
이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.
챕터
요약
Here we present a protocol to analyze the protein composition of large native protein:protein and protein:nucleic acid complexes from oilseed rape (B. napus) phloem exudate using a 3D polyacrylamide gel electrophoresis (PAGE) approach combining blue native (BN) with two denaturing PAGEs followed by mass spectrometric identification.