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JoVE Journal
Immunology and Infection

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Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies
 

Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies

Article DOI: 10.3791/57100-v 12:32 min March 13th, 2018
March 13th, 2018

챕터

요약

We describe here a method to isolate and purify dendritic cells from different anatomical compartments in the human female reproductive tract for the evaluation of their phenotypical and functional characteristics. This method can be adapted to isolate other immune cells or dendritic cells from other mucosal tissues.

Tags

Keywords: Dendritic Cells Female Reproductive Tract Tissue Digestion Phenotypical Functional Immune Cells Epithelial Sheets Enzymatic Digestion Cell Isolation
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