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1Center for Free Electron Laser Science,DESY, 2Department of Physics,University of Hamburg, 3Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation,University of Hamburg, 4The Hamburg Center for Ultrafast Imaging,University of Hamburg, 5Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI), 6European Molecular Biology Laboratory, EMBL c/o DESY, 7Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry
Chapters
- 00:04Title
- 00:38Polydimethylsiloxane (PDMS) Mold Fabrication and In Situ X-ray Chip Fabrication
- 04:59Fluid Delivery Access Ports and Surface Treatment
- 06:51Protein Preparation and Crystallization
- 08:51Results: Crystallization and In Situ X-ray Diffraction of Protein Crystals
- 10:41Conclusion
Este protocolo descreve em detalhes como fabricar e operar dispositivos microfluídicos para coleta de dados de difração de raios x em temperatura ambiente. Além disso, descreve como monitorar a cristalização de proteínas por difusão dinâmica da luz e como processar e analisar obtidos dados de difração.
