Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Dylan J. Stolz; Ethan M. Sands; Nansalmaa Amarsaikhan; Angar Tsoggerel; Steven P. Templeton

doi:10.3791/57155

Journal

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면역학 및 감염병학

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Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

DOI:

10.3791/57155-v

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09:52 min

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March 09, 2018

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Dylan J. Stolz, Ethan M. Sands, Nansalmaa Amarsaikhan, Angar Tsoggerel, Steven P. Templeton

¹Department of Microbiology and Immunology,Indiana University School of Medicine – Terre Haute

Chapters

00:05Title
01:04Aspergillus Fumigatus Conidia Infection
03:09Mouse Lung Harvest and Preservation
05:09Light Microscopy Imaging and Fungal Burden Calculation
07:26Results: Representative Survival, Fungal Burden, and Fungal Clearance in a Mouse Model of Invasive Aspergillus Infection
08:53Conclusion

Summary

자동 번역

Here we describe a protocol to determine pulmonary fungal burden in mice with invasive aspergillosis by quantification of Gomori's modified methanamine silver staining in histological sections. Use of this method resulted in comparable results with less animals compared to assessment of fungal burden by quantitative PCR of lung fungal DNA.

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Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

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