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Chapters
- 00:04Title
- 00:50Capsule Induction and Yeast Cell Staining
- 03:14Image Acquisition
- 04:27Capsule Measurement
- 06:33Results: Representative Capsule Measurement
- 07:58Conclusion
The polysaccharide capsule is the primary virulence factor in Cryptococcus neoformans, and its size correlates with strain virulence. Capsule diameter measurements are used in phenotypic testing and to gauge therapeutic efficacy. Here a standard method of capsule induction is presented, and two methods of staining and measuring diameter are compared.
