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Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver
DOI:

07:06 min

February 06, 2018

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Chapters

  • 00:05Title
  • 00:28Surgical Process
  • 03:06Intravital Imaging of the Liver with Two-photon MIcroscopy
  • 04:51Results: Two-photon Intravital Imaging in LPS-treated Mouse Liver
  • 06:03Conclusion

Summary

자동 번역

We established a novel surgical protocol for two-photon imaging of live mice liver with minimal invasion. With this technique, we identified the detailed structure of the liver during lipopolysaccharide-induced endotoxemia. We anticipate that this method may be utilized to determine the effectiveness of various reagents treatment to hepatic leukocyte migration.

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