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Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
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Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

DOI:
10.3791/57197-v

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09:47 min

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July 17, 2018

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,
1National Institute for Physiological Sciences, 2Graduate School of Science and Engineering,Saitama University

Chapters

  • 00:04Title
  • 00:43Synchronous Culture of Cyanobacteria and Monitoring by Fluorescence Microscopy
  • 02:31Sample Freezing and Cryo-HVET
  • 05:16Tomographic Reconstruction and Segmentation of the Feature of Interest
  • 07:20Results: Observations of Compacted DNA in Cyanobacteria
  • 08:36Conclusion

Summary

자동 번역

자동 번역

This protocol describes how to visualize the transient DNA compaction in cyanobacteria. Synchronous cultivation, monitoring by fluorescence microscopy, rapid freezing, and high voltage cryo-electron tomography are used. A protocol for these methodologies is presented, and future applications and developments are discussed.

Tags

DNA CompactionCyanobacteriaHigh-voltage Cryo-electron TomographyBacterial Cell CycleUltrastructureHoechst StainFluorescence MicroscopyPlunge-freezingLiquid NitrogenLiquid Ethane

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