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ๅคๆ‚ๆ ธๅž‹็ป†่ƒžๅ‘จๆœŸๆ•่Žท็ป†่ƒž็š„็”ŸๆˆไธŽๅˆ†็ฆป
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์•” ์—ฐ๊ตฌํ•™
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JoVE ์‹ ๋ฌธ ์•” ์—ฐ๊ตฌํ•™
Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
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ๅคๆ‚ๆ ธๅž‹็ป†่ƒžๅ‘จๆœŸๆ•่Žท็ป†่ƒž็š„็”ŸๆˆไธŽๅˆ†็ฆป

DOI:
10.3791/57215-v

โ€ข

05:22 min

โ€ข

April 13, 2018

โ€ข

, ,
1Koch Institute for Integrative Cancer Research at MIT, Department of Biology,Massachusetts Institute of Technology

Chapters

  • 00:04Title
  • 00:32Synchronization of RPE-1 hTERT Cells
  • 01:34Generation of Aneuploid Cells by Interference with the Activity of the Mitotic Kinase Mps1 and Removal of Aneuploid Cycling Cells to Enrich the ArCK Population
  • 03:36Results: In Vitro Isolation and Characterization of Aneuploid Cells with Complex Karyotypes (ArCK)
  • 04:35Conclusion

Summary

์ž๋™ ๋ฒˆ์—ญ

์ž๋™ ๋ฒˆ์—ญ

ไฝ“ๆฃ€ๅฏผ่‡ดๅŸบๅ› ็ป„ไธ็จณๅฎš, ๆœ€็ปˆไบง็”Ÿ็ป†่ƒžๅ‘จๆœŸ-่ขซๆ•่Žท็š„็ป†่ƒžไธŽๅคๆ‚็š„ๆ ธๅž‹ใ€‚ๆœฌๆ–‡ๆไพ›ไบ†ไธ€็ง็ฎ€ๅ•ๆ–นไพฟ็š„ๅˆ†็ฆปๅผ‚ๅ€ไฝ“็ป†่ƒž็š„ๆ–นๆณ•ใ€‚

Tags

Cell Cycle ArrestComplex KaryotypesAneuploid CellsCell SynchronizationRPE-1 HTERT CellsThymidineMps1 Inhibitor ReversineNocodazoleMitotic Shake-offArCK Cells

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