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光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响
 
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光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响

Article DOI: 10.3791/57223-v • 09:17 min • March 14th, 2018
March 14th, 2018 •
1, 1, 1
1Center for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia

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该协议提供了一个工作流, 如何将人工 microRNA 介导的 RNA 干扰与光遗传学相结合, 以刺激特定突触前 boutons 在完整的神经元回路中的选择性基因表达减少。

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神经科学 问题 133 光遗传学 超快 channelrhodopsin ChETA microRNA RNA 干扰 击倒 重组腺相关病毒 rAAV1/2 突触前 突触传递 突触可塑性 RNA 提取
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