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人胰岛自身抗体的电化学发光测定

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Electrochemiluminescence Assays for Human Islet Autoantibodies

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人胰岛自身抗体的电化学发光测定

DOI:

10.3791/57227-v

•

09:15 min

•

March 23, 2018

•

Yong Gu², Zhiyuan Zhao, Dongmei Miao, Hilary High, Tao Yang, Liping Yu

¹Barbara Davis Center for Childhood Diabetes,University of Colorado Denver, ²Department of Endocrinology,First Affiliated Hospital of Nanjing Medical University

Chapters

00:05Title
01:51Labeling of the Human Islet Autoantigen Followed by Incubation of the Serum Samples with the Labeled Antigens
05:01Preparation of the Streptavidin Plate, Transfer of the Serum/Antigen Incubate, and Reading of the Streptavidin Plate
06:32Results: Data Analysis
08:15Conclusion

Summary

자동 번역

在这里, 我们提出了检测人胰岛抗体的方法, 使用电化学发光 (ECL) 测定。该协议, 用于预测1型糖尿病, 可以扩大, 以检测自身抗体的其他自身免疫性疾病。

Tags

Electrochemiluminescence Islet Autoantibodies Type 1 Diabetes Autoimmune Non-radioactive Radioassay ELISA Insulin Autoantibody Biotin SULFO-TAG Spin Column Checkerboard Assay Phosphate Buffered Saline

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