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A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
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A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

DOI:
10.3791/57258-v

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09:22 min

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January 25, 2018

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,
1Department of Biology,California State University, Northridge

Chapters

  • 00:05Title
  • 00:57Protoplast Isolation
  • 04:09Protoplast Transformation
  • 06:00Protoplast Incubation and Harvesting
  • 06:46Results: Efficient Isolation of Soybean Protoplasts for Transient Gene Expression Studies
  • 08:28Conclusion

Summary

자동 번역

자동 번역

We developed a simple and efficient protocol for the preparation of large quantities of soybean protoplasts to study complex regulatory and signaling mechanisms in live cells.

Tags

Keywords: Soybean ProtoplastsTransient Gene ExpressionRegulatory NetworksProtein InteractionsUnifoliate LeavesEnzyme SolutionVacuum InfiltrationProtoplast DigestionW5 SolutionNylon MeshCentrifugation

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