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Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
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Chapters
- 00:04Title
- 01:26Growth of PA14NR Set Master Copy on LB Agar Plates
- 05:02Growth of the PA14NR Set Master Copy in LB Liquid Broth
- 07:01Transfer of PA14NR Set Overnight Cultures into Destination Plates
- 09:43Results: Protocol Generates High-quality Copies of the PA14NR Set Mutant Library
- 10:38Conclusion
Pseudomonas aeruginosa infection causes significant morbidity in vulnerable hosts. The nonredundant transposon insertion mutant library of P. aeruginosa strain PA14, designated as PA14NR Set, facilitates analysis of gene functionality in numerous processes. Presented here is a protocol to generate high-quality copies of the PA14NR Set mutant library.
