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Journal / Bioengineering / 凝胶序列: 一种同时测序库的方法用水凝胶基质制备 DNA 和…
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Bioengineering

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凝胶序列: 一种同时测序库的方法用水凝胶基质制备 DNA 和 RNA

Article DOI: 10.3791/57315-v • 09:19 min • March 26th, 2018
March 26th, 2018 •
*1, *2, 2, 3, 2
1Department of Engineering, University of San Diego, 2Department of Bioengineering, University of California, San Diego, 3Department of Mechanical and Aerospace Engineering, University of California, San Diego
* These authors contributed equally

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凝胶序列使研究人员能够同时为 DNA 和 RNA 的存储提供从 100-1000 细胞开始使用简单的水凝胶装置的可忽略的额外成本。本文给出了该装置的制作方法, 以及生成配对库的生物协议。

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生物工程 问题 133 电泳 聚丙烯酰胺水凝胶 下一代测序 基因组 转录 图书馆准备 DNA RNA
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