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Chapters
- 00:05Title
- 00:50Vesicle Preparation
- 04:43Vesicle Purification
- 06:59Use of Vesicles in the Presence of Magnesium
- 07:53Hammerhead RNA Self Cleavage in Vesicles
- 09:02Results: Hammerhead Ribozyme Cleavage in Oleic Acid/Glycerol Monooleate Vesicles
- 10:00Conclusion
Liposomes containing single-chain amphiphiles, particularly fatty acids, exhibit distinct properties compared to those containing diacylphospholipids due to the unique chemical properties of single chain amphiphiles. Here we describe techniques for the preparation, purification, and use of liposomes comprised in part or whole of these amphiphiles.
