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Una proyección basadas en células de islotes pancreáticos murinos Diabetogenic ambiental productos químicos
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals
DOI:

07:39 min

June 25, 2018

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Chapters

  • 00:04Title
  • 00:49Pancreas Perfusion and Removal
  • 01:43Pancreas Digestion and Purification of Islets
  • 03:30Plating and Treatment of Islet Cells
  • 04:23ROS Staining and Measurement
  • 05:28Results: Xenobiotic Treatment-induced Reactive Oxygen Species and Inhibition of Insulin Secretion in Isolated Pancreatic Islet Cells
  • 06:39Conclusion

Summary

자동 번역

Aquí presentamos un protocolo para aislar células de islote pancreático de ratón para la detección de las inducciones de ROS por los xenobióticos con el fin de identificar los potenciales químicos xenobióticos diabetogenic.

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