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1Department of Rheumatology, Ren Ji Hospital, School of Medicine,Shanghai Jiao Tong University, 2Department of Nephrology and Rheumatology,Yongchuan Hospital of Chongqing Medical University, 3Department of Medical Oncology, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College,Fudan University
Chapters
- 00:04Title
- 00:48Melanoma Cell-derived Factor Harvest and Bone Marrow-derived Adipocyte Induction
- 03:09Red Oil O Staining and Quantitative Polymerase Chain Reaction (qPCR) Analysis
- 04:20Bone Marrow Adipocyte-melanoma Cell Co-culture
- 05:54Results: Dynamic Bone Marrow-derived Adipocyte Regulation of Tumor Cells
- 06:41Conclusion
Here, we present a reliable and straightforward two-dimensional (2D) coculture system for studying the interaction between tumor cells and bone marrow adipocytes, which reveals a dual effect of melanoma cell-derived factors on the bone marrow adipocytes differentiation and also poses a classic method for the mechanistic study of bone metastasis.
