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Genetics

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JoVE Journal Genetics
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
 

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Article DOI: 10.3791/57350-v • 09:51 min • May 25th, 2018
May 25th, 2018 •
*1,2, *1, *3,4,5,6, *1,3, 1,3, 3,5,6,7,8,9, 1,2, 1,10, 3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
* These authors contributed equally

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요약
Automatic Translation

Utilizing a preassembled Cas9 ribonucleoprotein complex (RNP) is a powerful method for precise, efficient genome editing. Here, we highlight its utility across a broad range of cells and organisms, including primary human cells and both classic and emerging model organisms.

Tags

Genome Editing Cas9 Ribonucleoprotein CRISPR Targeted Genomic Changes Insertions Deletions Substitutions Model Organisms Non-model Organisms Efficiency Off-target Events Ex Vivo Therapies Cancer HIV Genetic Disease Harmful Mutations Primary Human Cells C.elegans Parhyale Hawaiensis Genetic Changes Guide RNA Cas9 Protein
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