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1Department of Molecular Cell Biology,University of California, Berkeley, 2Howard Hughes Medical Institute,University of California, Berkeley, 3Innovative Genomics Institute,University of California, Berkeley, 4Biomedical Sciences Graduate Program,University of California, San Francisco, 5Department of Microbiology and Immunology,University of California, San Francisco, 6Diabetes Center,University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine,University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center,University of California, San Francisco, 10Department of Integrative Biology,University of California, Berkeley
Chapters
- 00:04Title
- 01:09RNP Preparation for Primary Cell Editing, Hematopoietic Stem/Progenitor Cell (HSPC) Preparation, and RNP Electroporation
- 02:46C. elegans Gonad Microinjection with RNPs and Post-injection Care
- 05:17P. hawaiensis Embryo Microinjection with RNPs and Post-injection Care
- 08:05Results: Evaluation of Genome Editing Outcomes
- 09:28Conclusion
Utilisant un Cas9 préassemblé complexe ribonucléoprotéique (RNP) est une méthode puissante pour l’édition de génome précis et efficace. Ici, nous mettons en évidence son utilité dans un large éventail de cellules et des organismes, y compris des cellules humaines primaires et les deux classiques et de nouveaux organismes modèles.
