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Construction du bactériophage synthétique affiche Fab bibliothèque avec diversité sur mesure
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1Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University, 2Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto, 3Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto
Chapters
- 00:04Title
- 01:18Electroporation and Calculation of the Library Size
- 06:02Quality Assessment by Protein A/L Direct Binding ELISA Assay and Sequencing
- 09:43Results: Conversion of dU-ssDNA to CCC-DNA and Phage Titration for Calculation of Library Size
- 11:07Conclusion
Ce protocole décrit une procédure détaillée pour la construction d’une bibliothèque d’anticorps synthétiques phage-affiché avec diversité sur mesure. Anticorps synthétiques ont des applications larges depuis la recherche fondamentale pour le diagnostic de la maladie et la thérapeutique.
