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1Department of Coloproctological Surgery,Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis,Juntendo University Faculty of Medicine, 3Atopy Research Center,Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery,Tokyo Dental College, 5Department of Gastroenterology,Juntendo University Faculty of Medicine
Chapters
- 00:04Title
- 00:57Colorectal Cancer Cell (CRC) Organoid Artificial Extracellular Matrix Culture Installation
- 02:42CRC Organic Cell-labeling with GFP Lentiviral Particles
- 05:28GFP-labeled CRC Organoid Metastasis Generation
- 07:55Results: Representative High-resolution GFP Visualization in Cultured GFP-labeled CRC Organoids, Primary Tumors, and Micrometastases
- 08:38Conclusion
Om zeer gevoelige detectie van de verspreiden menselijke colorectaal carcinoom (CRC) cellen koloniseren van weefsels, tonen we hierin een protocol voor efficiënte transductie van groen fluorescent proteïne (GFP) lentivirale deeltjes in PDX-afgeleide CRC organoid cellen voorafgaand aan hun injectie in ontvangende muizen, met stereo-fluorescentie microscopische observatie.
