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GFP レンチ ウイルス導入オルガノイド培養患者由来大腸腫瘍によって生成される微小の高感度検出
JoVE 신문
암 연구학
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JoVE 신문 암 연구학
High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor
DOI:

09:16 min

June 14, 2018

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Chapters

  • 00:04Title
  • 00:57Colorectal Cancer Cell (CRC) Organoid Artificial Extracellular Matrix Culture Installation
  • 02:42CRC Organic Cell-labeling with GFP Lentiviral Particles
  • 05:28GFP-labeled CRC Organoid Metastasis Generation
  • 07:55Results: Representative High-resolution GFP Visualization in Cultured GFP-labeled CRC Organoids, Primary Tumors, and Micrometastases
  • 08:38Conclusion

Summary

자동 번역

CRC の PDX 由来器官毛細細胞に緑色蛍光タンパク質 (GFP) レンチ ウイルス粒子の効率的な伝達のためのプロトコルを示す本普及のひと大腸癌の高感度検出 (CRC) 細胞組織を植民地化をできるように、ステレオ蛍光顕微鏡による観察で、受信者のマウスに注入する前に。

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