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1Institute for Integrated Cell-Material Sciences (WPI-iCeMS),Kyoto University, 2Department of Life Science and Technology, School of Life Science and Technology,Tokyo Institute of Technology, 3Biomaterials Center for Regenerative Medical Engineering,Foundation for Advancement of International Science, 4Faculty of Science and Natural Resources,Universiti Malaysia Sabah, 5Institute for Chemical Research,Kyoto University, 6Ecole Normale Supérieure
Chapters
- 00:04Title
- 00:38Nanofiber Array Preparation
- 03:06Fabrication of Microfluidic Structures and Assembly of MACME Arrays
- 03:57Loading hESCs into MACME Array and Image Acquisition
- 05:23Results: Analysis of Multiplexed Artificial Cellular Microenvironments for Human Pluripotent Stem Cells
- 07:01Conclusion
I denne artikel beskrives den detaljerede metode til at forberede en multipleksede kunstige cellulære mikromiljø (MACME) array for høj overførselshastighed manipulation af fysiske og kemiske cues efterligner i vivo cellulære microenvironments og til identificere den optimal cellulære miljø for humane pluripotente stamceller (hPSCs) med enkelt-celle profilering.
