JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Institute for Integrated Cell-Material Sciences (WPI-iCeMS),Kyoto University, 2Department of Life Science and Technology, School of Life Science and Technology,Tokyo Institute of Technology, 3Biomaterials Center for Regenerative Medical Engineering,Foundation for Advancement of International Science, 4Faculty of Science and Natural Resources,Universiti Malaysia Sabah, 5Institute for Chemical Research,Kyoto University, 6Ecole Normale Supérieure
Chapters
- 00:04Title
- 00:38Nanofiber Array Preparation
- 03:06Fabrication of Microfluidic Structures and Assembly of MACME Arrays
- 03:57Loading hESCs into MACME Array and Image Acquisition
- 05:23Results: Analysis of Multiplexed Artificial Cellular Microenvironments for Human Pluripotent Stem Cells
- 07:01Conclusion
Denne artikkelen beskriver detaljert metodikk for å forberede en multiplekset kunstig Cellular MicroEnvironment (MACME)-matrise for høy gjennomstrømming manipulering av fysiske og kjemiske pekepinner mimicking i vivo mobilnettet microenvironments og til identifisere optimal mobilnettet miljø for menneskelig pluripotent stamceller (hPSCs) med encellede profilering.
