Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Adeno-associated Virus-mediated Transgene…
JoVE Journal
Neuroscience

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Neuroscience
Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
 

Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord

Article DOI: 10.3791/57382-v • 08:41 min • May 12th, 2018
May 12th, 2018 •
*1,2, *1, 1, 1
1Institute of Pharmacology and Toxicology, University of Zurich, 2Institute of Pharmaceutical Sciences, Swiss Federal Institute (ETH) Zurich
* These authors contributed equally

챕터

요약
Automatic Translation

Intraspinal injection of recombinase dependent recombinant adeno-associated virus (rAAV) can be used to manipulate any genetically labelled cell type in the spinal cord. Here we describe how to transduce neurons in the dorsal horn of the lumbar spinal cord. This technique enables functional interrogation of the manipulated neuron subtype.

Tags

Keywords: Adeno-associated Virus Transgene Expression Genetically Defined Neurons Spinal Cord Pain Perception Intraspinal Delivery Stereotaxic Surgery Vertebrae Identification
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter