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En Protein Microarray analys för serologisk bestämning av Antigen-specifika antikroppen Svaren följande Clostridium difficile infektion
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1Breast Surgery Group, Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, Queen’s Medical Centre,University of Nottingham, 2Medical Microbiology and Immunology Department, Faculty of Medicine,Mansoura University, 3Nottingham Digestive Diseases Centre and NIHR Nottingham Digestive Diseases Biomedical Research Centre, School of Medicine, Queen’s Medical Centre,University of Nottingham
Chapters
- 00:04Title
- 01:14Preparing a Microarray Plate and Printing with a Contact Robot
- 03:06Array Probing
- 05:19Array Scanning
- 06:36Results: Validation of Protein Microarray to Detect Isotype Specific Antibody Response to Clostridium difficile Toxins and Its Reproducibility
- 08:01Conclusion
Denna artikel beskriver en enkel protein microarray metod för profilering humorala immunsvar mot en 7-plex panel av mycket renad Clostridium difficile antigener i humant serum. Protokollet kan förlängas för bestämning av specifika antikroppen svaren i förberedelserna av polyklonala intravenöst immunglobulin.
