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Chapters
- 00:04Title
- 00:47Pre-sensitization of Cells
- 02:03Micro-irradiation of Cells
- 04:27Recruitment Kinetics Analysis
- 05:50IF Protocol
- 09:05Results: Analysis of Protein Recruitment to DNA Lesions Using 405 nm Laser Micro-irradiation
- 10:59Conclusion
Studying DNA damage repair kinetics requires a system to induce lesions at defined sub-nuclear regions. We describe a method to create localized double-stranded breaks using a laser-scanning confocal microscope equipped with a 405 nm laser and provide automated procedures to quantify the dynamics of repair factors at these lesions.
