Journal
/
신경과학
/
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
JoVE 신문
신경과학
This content is Free Access.
JoVE 신문 신경과학
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

DOI:
10.3791/57431-v

•

09:20 min

•

April 27, 2018

•

, ,
1Centre for Discovery Brain Sciences,University of Edinburgh

Chapters

  • 00:04Title
  • 01:05Homogenization
  • 04:40Microglia Immunostaining
  • 06:41Results: Isolation of Neurons, Macrophages, and Microglia, and their RNA from Larval Zebrafish Brains
  • 08:25Conclusion

Summary

자동 번역

자동 번역

We present a protocol to isolate neurons, macrophages and microglia from larval zebrafish brains under physiological and pathological conditions. Upon isolation, RNA is extracted from these cells to analyze their gene expression profile. This protocol allows for the collection of high-quality RNA for performing downstream analysis like qPCR and transcriptomics.

Tags

IsolationRNA ExtractionNeuronsMacrophagesMicrogliaLarval Zebrafish BrainsTransgenic ZebrafishLive ImagingGene ExpressionCell SortingCentral Nervous SystemPTUFluorescent StereomicroscopeGFPDsREDTricaineHomogenizerMedia ARNA PurificationQPCRTranscriptomics

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/57431?article_type=v

Isolation and Culture of Hippocampal Neurons from Prenatal Mice
check_url/kr/57431?article_type=v

Production and Isolation of Axons from Sensory Neurons for Biochemical Analysis Using Porous Filters
check_url/kr/57431?article_type=v

Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos
check_url/kr/57431?article_type=v

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
check_url/kr/57431?article_type=v

Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains
check_url/kr/57431?article_type=v

Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells
check_url/kr/57431?article_type=v

Spinal Cord Neurons Isolation and Culture from Neonatal Mice
check_url/kr/57431?article_type=v

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
check_url/kr/57431?article_type=v

Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains
check_url/kr/57431?article_type=v

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Read Article