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小鼠卵母细胞在减数分裂过程中对人类基因功能的评估

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Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

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小鼠卵母细胞在减数分裂过程中对人类基因功能的评估

DOI:

10.3791/57442-v

•

11:13 min

•

April 10, 2018

•

Diego Marin*^1,2, Alexandra L. Nguyen*³, Richard T. Scott, Jr.², Karen Schindler

¹IVI-RMA New Jersey, ²Jefferson College of Biomedical Sciences,Thomas Jefferson University, ³Department of Genetics,Rutgers, The State University of New Jersey

Chapters

00:04Title
01:00Molecular Cloning
02:23Kinetochore-microtubule Attachment Assay
04:57Chromosome Alignment Challenge Assay
08:52Results: Assessment of Human Gene Function During Meiosis in Mouse Oocytes
10:12Conclusion

Summary

자동 번역

随着与人类疾病相关的遗传变异开始显露出来, 开发系统以迅速评估那些被确定的变种的生物学意义变得越来越重要。本协议描述了在女性减数分裂过程中使用小鼠卵母细胞评估人类基因功能的方法。

Tags

Keywords: Mouse Oocytes Human Gene Function Meiosis I Maternal Aneuploidy In Vitro Fertilization Exome Sequencing Cloning Mutagenesis In Vitro Transcription Microinjection Milrinone Metaphase I

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