A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:40Annealing of vDNA
- 02:35Intasome Assembly
- 04:12Intasome Solubilization and Purification
- 06:23Integration Strand Transfer Assay, Fraction Selection, and Storage
- 08:29Results: Isolation and Evaluation of PFV Intasomes
- 09:45Conclusion
Rekombinant retroviral integrase og DNA oligomerer efterligne viral DNA ender kan danne en enzymatisk aktive kompleks kendt som en intasome. Intasomes kan anvendes til biokemiske, strukturelle og kinetiske studier. Denne protokol detaljer om, hvordan at samle og rense prototype skummende virus intasomes.
