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MALDI 质谱法测定小鼠组织提取物蛋白酶特异性: 操作 PH 值导致特异性改变
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The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
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MALDI 质谱法测定小鼠组织提取物蛋白酶特异性: 操作 PH 值导致特异性改变

DOI:
10.3791/57469-v

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09:47 min

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May 25, 2018

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, ,
1Department of Microbiology and Molecular Cell Biology,Nihon Pharmaceutical University, 2Department of Clinical Pharmaceutics,Nihon Pharmaceutical University

Chapters

  • 00:05Title
  • 00:36Preparation of Tissue Extracts
  • 01:40Digestion of Substrates in Model Protease and Tissue Extracts
  • 02:59Purification of the Iminobiotin-labeled Substrate
  • 05:17Sample Preparation and Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF Mass Spectrometry)
  • 06:48Results: Identification of Protease Specificity
  • 08:46Conclusion

Summary

자동 번역

자동 번역

在这里, 我们提出了一个协议, 以确定蛋白酶特异性的粗鼠组织提取物使用 MALDI 的光谱。

Tags

Protease SpecificityMALDI-TOF Mass SpectrometryPH ManipulationProtease AssayBiotin LabelingStreptavidin Beads

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