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MicroRNA 移植成人骨髓造血干细胞促进细胞工程与磁性靶向结合的协议
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Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting
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MicroRNA 移植成人骨髓造血干细胞促进细胞工程与磁性靶向结合的协议

DOI:
10.3791/57474-v

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11:37 min

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June 18, 2018

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, , , ,
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery,Rostock University Medical Center, 2Department Life, Light and Matter of the Interdisciplinary Faculty,Rostock University

Chapters

  • 00:04Title
  • 00:53CD133 SC Isolation
  • 06:00Characterization of Freshly Isolated CD133 SCs
  • 07:21Preparation of Transfection Complexes
  • 08:26CD133 SCs Transfection and Analysis
  • 10:03Results: Successful Engineering of miR-loaded Magnetized CD133 Cells
  • 11:02Conclusion

Summary

자동 번역

자동 번역

该协议说明了一个安全和有效的程序, 以修改 CD133+造血干细胞。所提出的非病毒, 磁性 polyplex 公司的方法可以为优化治疗干细胞效应, 并通过磁共振成像监测所管理的细胞产品提供了依据。

Tags

MicroRNA TransferAdult Bone Marrow-derived Hematopoietic Stem CellsCell EngineeringMagnetic TargetingRegenerative MedicineHematopoietic Stem Cell ModificationMesenchymal Stem CellsBone Marrow CollectionDensity Gradient CentrifugationMononuclear Cell IsolationMagnetic Selection

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