Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections

Yuka Mizutani; Daisuke Kuga; Machiko Iida; Kaori Ushida; Tsuyoshi Takagi; Yoshihito Tokita; Masahide Takahashi; Masato Asai

doi:10.3791/57475

Journal

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생물학

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抗磷 girdin 抗体在自由漂浮小鼠空肠 Cryosections 小肠簇细胞中的应用

JoVE 신문
생물학

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JoVE 신문 생물학

Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections

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抗磷 girdin 抗体在自由漂浮小鼠空肠 Cryosections 小肠簇细胞中的应用

DOI:

10.3791/57475-v

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06:26 min

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March 21, 2018

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Yuka Mizutani, Daisuke Kuga, Machiko Iida, Kaori Ushida, Tsuyoshi Takagi, Yoshihito Tokita, Masahide Takahashi, Masato Asai³

¹Division of Perinatology, Institute for Developmental Research,Aichi Human Service Center, ²Surgery Department,Anjo Kosei Hospital, ³Department of Pathology,Nagoya University Graduate School of Medicine

Chapters

00:04Title
00:40Dissection and Isolation of Jejunum
02:40Immunofluorescence of Tuft Cells Using Free-floating Cryosections
04:42Results: Representative Fluorescence Images of Mouse Intestinal Tuft Cells
05:55Conclusion

Summary

자동 번역

库加et . 发现在酪氨酸 1798 (pY1798) 中, 抗肌动蛋白结合蛋白 girdin 磷酸化的特定抗体可用于标记一簇细胞 (TCs)。该协议允许使用 pY1798 抗体的自由漂浮空肠 cryosections 免疫荧光染色对 TCs 进行鲁棒可视化。

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