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提耳伸肌压钳条件下哺乳动物神经肌肉传输的检查准备

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Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions

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提耳伸肌压钳条件下哺乳动物神经肌肉传输的检查准备

DOI:

10.3791/57482-v

•

10:45 min

•

May 05, 2018

•

Steven R. A. Burke, Eric J. Reed, Shannon H. Romer, Andrew A. Voss

¹Department of Biological Sciences,Wright State University

Chapters

00:04Title
01:18Levator Auris Longus Muscle Isolation
05:22Isolation of Nerve
06:50Electrophysiology Experiment
08:00Identification of Neuromuscular Junction using Fluorescence
08:54Results: Current-clamp and Voltage-clamp Recording of Membrane Properties
09:42Conclusion

Summary

자동 번역

本文所描述的协议使用鼠标提耳肌 (拉尔) 肌肉记录自发性和神经诱发突触后电位 (电流钳) 和电流 (电压钳) 在神经肌肉连接。使用该技术可以为正常和疾病条件下突触传递机制提供关键的见解。

Tags

Levator Auris Longus Neuromuscular Transmission Voltage Clamp Synaptic Currents End Plate Current Quantal Content Probability Of Release Neurotransmission Muscle Excitability Synaptic Function Nerve-muscle Preparation Mammalian Dissection Electrophysiology

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