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Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts
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Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts
Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts
DOI:
10.3791/57490-v
•
09:20 min
•
June 26, 2018
•
Jin-Young Lee*
1
,
Aloran Mazumder*
1
,
Marc Diederich
1
Department of Pharmacy, College of Pharmacy
,
Seoul National University
Chapters
00:04
Title
00:33
Colony Formation Assays
03:47
Zebrafish Egg Production and Incubation for Xenograft Assay
05:05
Compound-treated Cell Preparation and Microinjection of Cancer Cells for Xenograft Formation
07:21
Results: Inhibitory Potential of Anticancer Compounds on Spheroid Formation and
In Vivo
Tumor Formation in Zebrafish
08:29
Conclusion
Summary
자동 번역
English (Original)
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자동 번역
Here, we present the preclinical screening of anticancer coumarins using 3D culture and zebrafish.
Tags
Keywords: Preclinical Assessment
Bioactivity
Anticancer Coumarin OT48
Spheroids
Colony Formation Assays
Zebrafish Xenografts
In Vitro
In Vivo
3D Culture Systems
A549 Cells
RPMI 1640 Medium
Methylcellulose-based Medium
MTT Assay
ImageJ Software
Drug Discovery
Article
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