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Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts
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Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts

Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts

DOI:
10.3791/57490-v

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09:20 min

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June 26, 2018

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, ,
1Department of Pharmacy, College of Pharmacy,Seoul National University

Chapters

  • 00:04Title
  • 00:33Colony Formation Assays
  • 03:47Zebrafish Egg Production and Incubation for Xenograft Assay
  • 05:05Compound-treated Cell Preparation and Microinjection of Cancer Cells for Xenograft Formation
  • 07:21Results: Inhibitory Potential of Anticancer Compounds on Spheroid Formation and In Vivo Tumor Formation in Zebrafish
  • 08:29Conclusion

Summary

자동 번역

자동 번역

Here, we present the preclinical screening of anticancer coumarins using 3D culture and zebrafish.

Tags

Keywords: Preclinical AssessmentBioactivityAnticancer Coumarin OT48SpheroidsColony Formation AssaysZebrafish XenograftsIn VitroIn Vivo3D Culture SystemsA549 CellsRPMI 1640 MediumMethylcellulose-based MediumMTT AssayImageJ SoftwareDrug Discovery

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